Effect of Exogenous Application of Sodium Nitroprusside on Alleviation of Low Temperature Stress in Kimchi Cabbage (Brassica rapa ssp. pekinensis)

Jinhyoung Lee; Seunghwan Wi; Hyejin Lee; Sanggyu Lee; Minseo Kang; Taeyang Kim; Seonghoe Jang; Heeju Lee

doi:10.12791/KSBEC.2023.32.4.377

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2023 Vol.32, Issue 4 Preview Page Next Page

Original Articles

Effect of Exogenous Application of Sodium Nitroprusside on Alleviation of Low Temperature Stress in Kimchi Cabbage (Brassica rapa ssp. pekinensis) Sodium Nitroprusside 처리가 배추의 저온 스트레스 경감에 미치는 영향

31 October 2023. pp. 377-383

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Abstract

The effects of exogenous sodium nitroprusside (SNP, nitric oxide donor) on the growth, yield, photosynthetic characteristics, and antioxidant enzyme activity of kimchi cabbage (Brassica rapa L. subsp. pekinensis (Lour.) Hanelt) was studied under the low temperature conditions. Kimchi cabbages were treated with SNP of three concentrations (7.5, 15, 30 mg·L^-1) for three times at four-day intervals and exposed to low temperature (16/7°C) stress for seven days. SNP treatment induced increases of net photosynthetic rate (Pn), stomatal conductance (Gs), intracellular CO₂ concentration (Ci) and transpiration rate (Tr) under the stress condition with the highest level after the third treatment. The contents of malondialdehyde (MDA) and H₂O₂ were significantly lower in the treatment of SNP compared to the non-treated control. The activity of ascorbate peroxidase(APX), catalase (CAT), peroxidase (POD) and superoxide dismutase (SOD), increased in treated plants by up to 38, 187, 24 and 175%, respectively compared to the non-treated control. SNP-treated and untreated plants had similar growth characteristics. Compared to the control group, SNP-treatment increased fresh weight and leaf area by 5%. Overall, our findings suggest that the application of sodium nitroprusside to the leaves contributes to reducing physiological damage and enhancing the activities of antioxidant enzymes, thereby improving low temperature stress tolerance in kimchi cabbage.

Keywords

antioxidant enzyme

nitric oxide

oxidative stress

photosynthesis

본 연구에서는 저온 스트레스 조건에서 sodium nitroprusside (SNP, 산화질소 donor)의 경엽처리가 배추의 생장, 광합성 특성 및 생리활성 반응에 미치는 영향을 구명하고자 하였다. SNP를 각각 7.5, 15, 및 30mg·L^-1농도로 주당 100mL을 4일 간격으로 3회 엽면 살포하였고, 7일간 저온 스트레스를 처리하였다. SNP 처리 시 광합성 속도, 기공전도도, 세포 내 CO₂ 농도 및 증산 속도는 무처리 대비 증가하였고, 3회차 처리 후 가장 높았다. MDA와 H₂O₂ 함량은 무처리 대비 현저하게 감소하였다. APX, CAT, POD 및 SOD 활성은 무처리 대비 현저하게 증가하였으며, 각각 최대 38, 187, 24 및 175% 증가하였다. 배추의 생육 특성은 무처리구와 유의한 차이를 보이지 않았으나, 생체중과 엽면적은 5% 정도 증가하였다. 따라서 SNP의 경엽처리는 배추의 생육, 광합성 특성 및 항산화효소 활성을 증대시키고 저온 스트레스에 의한 피해 경감에 긍정적인 효과를 유발함을 확인하였다.

키워드

항산화효소

산화질소

산화 스트레스

광합성

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Information

Publisher :The Korean Society for Bio-Environment Control
Publisher(Ko) :(사)한국생물환경조절학회
Journal Title :Journal of Bio-Environment Control
Journal Title(Ko) :생물환경조절학회지
Volume : 32
No :4
Pages :377-383
Received Date : 2023-10-13
Revised Date : 2023-10-23
Accepted Date : 2023-10-24
DOI :https://doi.org/10.12791/KSBEC.2023.32.4.377

Journal of Bio-Environment Control ISSN:1229-4675(Print) 2765-3641(Online) 생물환경조절학회지

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